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详细说明

HiPerFect Transfection Reagent/תȾ

۸
3560
收藏
Ʒ˵
Ŀ¼ۣԪ
3017040.5ml1990
3017051ml3560
3017074  1ml12680


ϸsiRNAmiRNAתȾ

  • ŨµsiRNAЧתȾ

  • ЧתȾԭϸϸʸ

  • ЧתȾϸ;ϸ

  • TransFect Protocol Databaseϸ͵ʵ鷽

  • ЧתȾmiRNAģƼ

        HiPerFect Transfection ReagentһӺ֬ɵĶػܹЧsiRNAЧͷϸڲsiRNAʹʹõŨȵsiRNAҲɸЧó򡣳siRNAHiPerFect Transfection Reagentڵͨ͸ͨתȾmiRNAģƼTransFect Protocol Databaseϸ͵ʵ鷽

1_5.jpeg
ʹHiPerFect ReagentЧתȾNHEK
˲㵰A/CsiRNAAllStars Negative Control siRNAתȾ̥γϸ48Сʱ󣬲real-time RT-PCR˲㵰A/Cı


1_5 (1).jpeg
HiPerFect ReagentܹЧóCDC2
ʹQIAGENHiPerFect Transfection ReagentӦ̵Reagent LͬŨȵİCDC2siRNAתȾHeLa S3ϸڡͬʱתȾɫӫ⵰ףGFPķdzĬsiRNA48Сʱ󣬲öreal-time RT-PCRCDC2óˮƽGAPDHıCDC2ıб׼δתȾĶϸıˮƽͼʾ
1_5 (2).jpeg
ʹHiPerFect Transfection ReagentзתȾ
ڷתȾʵ鷽УϸĽתȾͬһڽСsiRNA/miRNAڣȻHiPerFect Reagentڸγɺ󣬽ϸڡЩתȾٱݣʵԶҳڸͨʵ顣


Performance

ϸϵԼֻδֻľϸѽй⣬ͨʹHiPerFect ReagentsiRNAתȾ⡣ϸϵɳɹתȾЧüл1ʹHiPerFect ReagentתȾһЩ紩תȾϸ˵ѡ


1.ʹHiPerFect ReagentɹתȾϸϵ
ϸϵϸsiRNAŨü
K562԰Ѫ5 nM85%
Jurkat75 nM83%
D1.150 nM84%
RAW 264.7Сϸ25 nM77%
J774.A1Сϸ50 nM97%
PMA-differentiated THP-1˼ԵϸѪ5 nM82%
MELѪ5-20 nM50-70%

HiPerFect ReagentʺתȾϸϵ2

2.HiPerFect ReagentʺתȾϸϵ
ϸϵϸ
Raji
U937˾ϸ
Molt4
Molt14
HL60ϸϵ
E6.1

siRNAŨ1C50 nM֮ʱЧó>80%μ"HiPerFect Reagent provides effective CDC2 knockdown"ʹ1 nM siRNAתȾóΪ86%5 nM siRNAתȾóʿɴ96%ѵsiRNAʹŨΪ1 nMٵѰЧӦЧóμ"Efficient transfection of NHEK"5 nMߵóЧʣ

Principle

siRNAתȾܷѰЧӦsiRNAsӰͬԴ򲿷ͬԴлıں̶ܴϿͨʹõŨȵsiRNA⡣ʹHiPerFect Transfection ReagentɸЧתȾͳĬijЩ¿ʹ10 pM siRNAʹHiPerFect Transfection ReagentںܿsiRNAŨȷΧڸЧתȾsiRNAʹоѡsiRNAŨȡתȾ͵ϸHeLaHeLa S3HEK 293NIH/3T3Huh-7HepG2MCF-7HUVECNHLF

miRNAо

micro RNAsmiRNAsһԴСRNAsiRNAsصͬmiRNAsڶҪãֻ͵תȾϳɵmiRNAģƼһڽضmiRNAsİбõļmiRNAģһֻѧϳɵmiRNAsģתȾϸȻmiRNAsmiRNAƼһֵRNAsܹmiRNAĹܡתȾmiRNAģƻıתȾmiRNAƼǿıɴ֤ĿmiRNAڸûıңmiRNAsڸźͨ·еҲͨתȾmiRNAģƼԵĻȷɸʵҲͨתȾmiRNAģĻСHiPerFect Transfection ReagentϸsiRNAĸЧתȾҲmiRNAģƼĵͨͨתȾ

Procedure

HiPerFect Transfection ReagentһּҺֻ轫ϡ͵siRNA/miRNAҺϡתƵϸɡתȾѪ½УȥϸеʡHiPerFect Transfection Reagent Handbookеʵ鷽TransFect Protocol Databaseҵʺϸͺƿ/ʵ鷽ݿṩȷеʵ鷽ǵеʵ鷽ʡʱ;ֻϸ͡ƿͼɴӡQIAGENתȾʵ鷽PDFʽTransFect Protocol Databaseѵģעᡣ

HiPerFect Transfection ReagentתȾʹóתȾ裬תȾǰ24СʱϸͬʱҲṩʹ96װ384װ巴תȾʵ鷽HiPerFect Transfection Reagent HandbookЩʵ鷽ͬһϸͽתȾHiPerFect Reagent󣬽siRNA/miRNAСϾȺ󣬽ϸУμ"Reverse transfection using HiPerFect Transfection Reagent"תȾʵ鷽١㡢ʵԶͨڸͨʵ顣

Applications

ʹsiRNAŨȽϵͣHiPerFect Transfection ReagentҲܹЧsiRNAתȾʺ϶Ӧã磺

  • RNAо

  • miRNAо

  • ͹о


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